Sperm recovery thecniques

what does it consist of?

In patients with certain types of azoospermia, or who have previously undergone a vasectomy, a sufficient amount of sperm cannot be found in a semen sample to perform an assisted reproduction treatment. In these cases, sperm recovery techniques allow to recover a sperm sample directly from the testicles.


  • PESA: Percutaneous Epididymal Sperm Aspiration. A needle is inserted under the skin of the scrotum to reach the epididymis and remove the sperm.
  • MESA: Microsurgical Epididymal Sperm Aspiration. A small incision, of one or two centimetres, is performed in the scrotal tissue to facilitate access to the epididymis. This method allows recovery of a greater volume of motile sperm.
  • TESA (Testicular sperm aspiration): fluid containing the sperm is aspirated from the testes using a needle.


  • TESE (Testicular sperm extraction): if the previous techniques have been ineffective, a biopsy of the testicle will be needed. To do this, an incision will be made in the scrotum and a portion of testicular tissue will be removed, which will contain the sperm.

who is it for?


Patients who have undergone a vasectomy


Patients with obstructive azoospermia (male whom produce sperm, however there is absence of spermatozoa in ejaculate)

Cystic fibrosis

Men with cystic fibrosis